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The Ion Torrent PGM instrument represents a recently commercialized bench-top NGS platform and is marketed as being less costly and with a faster turnaround as compared to other NGS techniques such as the 454 and Illumina platforms. The capability of high through-put sequencing of 16S rRNA gene sequences by means of Next Generation Sequencing (NGS) technologies has been pivotal in facilitating the discovery of gut microbiota biodiversity. In fact, (gut) disorders such as inflammatory bowel disease, , irritable bowel syndrome, obesity, and necrotizing enterocolitis have been considered as deviations from a healthy gut microbiota composition. During the last decade extensive efforts have been made to determine the complexity of the microbial communities residing in the gut (for review see ). The gut microbiota is presumed to play a key role in human health and disease through its impact on nutrition, pathogenesis and immunology. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors. There are no patents, products in development or marketed products to declare.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: GenProbio srl provided the financial support of the Laboratory of Probiogenomics for this study. Arancha Hevia was the recipient of an FPI grant, and Borja Sánchez was the recipient of a Juan de la Cierva postdoctoral contract. The authors also acknowledge the support of the Spanish Plan Nacional de I+D (grant AGL2010-14952). DvS is a member of The Alimentary Pharmabiotic Centre, which is a Centre for Science and Technology (CSET) funded by the Science Foundation Ireland (SFI), through the Irish Government’s National Development Plan (Grant no.
fellowship (Spinner 2013, Regione Emilia Romagna) to S.D. This work was also financially supported by a Ph.D. This work was financially supported by Fondazione Cariparma to MV and by a FEMS Advanced Fellowship 2011 and an IRCSET Embark postdoctoral fellowship to FT. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: The authors thank GenProbio srl for the financial support of the Laboratory of Probiogenomics. Received: JanuAccepted: JPublished: July 15, 2013Ĭopyright: © 2013 Milani et al. PLoS ONE 8(7):Įditor: Christopher Quince, University of Glasgow, United Kingdom (2013) Assessing the Fecal Microbiota: An Optimized Ion Torrent 16S rRNA Gene-Based Analysis Protocol. Every choice the player makes as Michael Thorton will carry consequences for his future and the fate of the world.Citation: Milani C, Hevia A, Foroni E, Duranti S, Turroni F, Lugli GA, et al.
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As players determine how to accomplish different objectives, the decisions made and actions taken in each mission will ultimately transform the type of secret agent Michael Thorton will become. To do so means he must cut himself off from the very people he is sworn to protect. A talented young agent cast out by his government, Thorton is the only one with the information needed to stop an impending international catastrophe. Loyalty carries a price and no one knows this more than agent Michael Thorton.
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